产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

GRP78 polyclonal antibody

货号: BS6479

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS6479

Product Name :GRP78 polyclonal antibody

Swiss-Prot :P11021

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:2000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :The HSP 70 family comprises four highly conserved proteins, HSP 70, HSC 70, GRP 75 and GRP 78, which serve a variety of roles. They act as molecular chaperones facilitating the assembly of multi-protein complexes, participate in the translocation of polypeptides across cell membranes and to the nucleus, and aid in the proper folding of nascent polypeptide chains. HSC 70, GRP 75 and GRP 78 are constitutively expressed in primate cells. HSP 70 expression is strongly induced in response to heat stress. HSP 70 and HSC 70, which are found in both the cytosol and nucleus of mammalian cells, play key roles in the cytosolic endoplasmic reticulum and mitochondrial import machinery.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :GRP78 polyclonal antibody detects endogenous levels of GRP78 protein.

BiowMW :~ 78 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :78 kDa glucose-regulated protein; GRP-78; Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Heat shock 70 kDa protein 5; Immunoglobulin heavy chain-binding protein; BiP; HSPA5; GRP78

Immunogen :Recombinant full length Human GRP7.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using BiP/GRP78 antibody at 1:1000 dilution.

Immunohistochemistry of paraffin-embedded rat brain using BiP/GRP78 antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human liver cancer using BiP/GRP78 antibody at dilution of 1:100 (40x lens).

Confocal immunofluorescence analysis of Hela cells using BiP/GRP78 antibody at dilution of 1:200. Blue: DAPI for nuclear staining.

The reproductive toxicology of male SD rats after PM2.5 exposure mediated by the stimulation of endoplasmic reticulum stress

JournalChemosphere

ApplicationWB

IF5.108

SpeciesRat

PMID28961540
mTORC1 activation downregulates FGFR3 and PTH/PTHrP receptor in articular chondrocytes to initiate osteoarthritis

JournalOsteoarthritis And Cartilage

ApplicationIHC

IF4.879

SpeciesMouse

PMID28043938
miR-663 overexpression induced by endoplasmic reticulum stress modulates hepatocellular carcinoma cell apoptosis via transforming growth factor beta 1

JournalOncoTargets and Therapy

ApplicationWB

IF4.147

SpeciesHuman

PMID27073326
Lipopolysaccharide markedly changes glucose metabolism and mitochondrial function in the longissimus muscle of pigs

JournalAnimal

ApplicationWB

IF3.24

SpeciesPiglets

PMID26863995
Endoplasmic Reticulum Stress May Not Be Involved in Intestinal Epithelial Cell Apoptosis in Experimental Acute Pancreatitis

JournalDigestive Diseases And Sciences

ApplicationIHC

IF2.937

SpeciesRat

PMID25616612