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[KO Validated] MEK1 polyclonal antibody

货号: BS6471

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS6471

Product Name :[KO Validated] MEK1 polyclonal antibody

Swiss-Prot :Q02750

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC

Application_all :WB,1:1000 - 1:3000|IHC,1:50 - 1:200

Background :The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :45kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :CFC3;MAPKK1;MEK1;MKK1;PRKMK1;MAP2K1

Immunogen :A synthetic peptide of human MEK1(NP_002746.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using MEK1 antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit .
Exposure time: 90s.

Western blot analysis of extracts from normal and MEK1 knockout HeLa cells, using MEK1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 5s.

Immunohistochemistry of paraffin-embedded Rat brain using MEK1 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded mouse kidney using MEK1 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

MicroRNA-296-5p increases proliferation in gastric cancer through repression of Caudal -related homeobox 1

JournalOncogene

ApplicationWB

IF6.634

SpeciesHuman

PMID23353818
MEK1–ERKs signal cascade is required for the replication of Enterovirus 71 (EV71)

JournalAntiviral Research

ApplicationWB

IF4.101

SpeciesHuman

PMID22101247