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RhoA polyclonal antibody

货号: BS6470

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS6470

Product Name :RhoA polyclonal antibody

Swiss-Prot :P61586

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IP

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200|IP,1:50 - 1:200

Background :This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants have been identified.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :21KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :ARH12;ARHA;RHO12;RHOH12;Rho;RHOA;RhoA

Immunogen :A synthetic peptide of human RhoA(NP_001655.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using RhoA antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit .
Exposure time: 180s.

Immunohistochemistry of paraffin-embedded human placenta using RhoA Rabbit pAb at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunoprecipitation analysis of 200ug extracts of NIH/3T3 cells using 3ug RhoA antibody . Western blot was performed from the immunoprecipitate using RhoA antibody at a dilution of 1:500.

Lymphatic endothelial cell‐secreted CXCL1 stimulates lymphangiogenesis and metastasis of gastric cancer

JournalInternational Journal Of Cancer

ApplicationWB

IF4.982

SpeciesRat

PMID21387301
ERK-MAPK Signaling Opposes Rho-Kinase to Reduce Cardiomyocyte Apoptosis in Heart Ischemic Preconditioning

JournalMolecular Medicine

ApplicationWB

IF2.991

SpeciesRat

PMID20383434
Rhos and Rho kinases in the rat prostate: their possible functional roles and distributions

JournalMolecular And Cellular Biochemistry

ApplicationWB,IHC

IF2.795

SpeciesRat

PMID
Effect of ART1 on the proliferation and migration of mouse colon carcinoma CT26 cells in vivo

JournalMolecular Medicine Reports

ApplicationWB

IF1.851

SpeciesMouse

PMID28138708
Opticin Ameliorates Hypoxia-Induced Retinal Angiogenesis by Suppression of Integrin α2-I Domain–Collagen Complex Formation and RhoA/ROCK1 Signaling

JournalInvestigative Ophthalmology & Visual Science

ApplicationWB

IF4.799

SpeciesZebrafish

PMID35006271