产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

CDKN1B/p27KIP1 polyclonal antibody

货号: BS6465

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS6465

Product Name :CDKN1B/p27KIP1 polyclonal antibody

Swiss-Prot :P46527

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:20 - 1:50

Background :This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4).

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :27kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :CDKN1B;CDKN4;KIP1;MEN1B;MEN4;P27KIP1;p27 KIP 1

Immunogen :Recombinant fusion protein of human CDKN1B/p27KIP1(NP_004055.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using CDKN1B/p27KIP1 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 3min.

Immunofluorescence analysis of NIH/3T3 cells using p27 KIP 1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using p27 KIP 1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

HOXB7 as a Prognostic Factor and Mediator of Colorectal Cancer Progression

JournalClinical Cancer Research

ApplicationWB

IF12.531

SpeciesHuman

PMID21474578
FOXC2 promotes colorectal cancer proliferation through inhibition of FOXO3a and activation of MAPK and AKT signaling pathways

JournalCancer Letters

ApplicationWB

IF7.36

SpeciesHuman

PMID25069037
MiR-196a Is Upregulated in Gastric Cancer and Promotes Cell Proliferation by Downregulating p27kip1

JournalMolecular Cancer Therapeutics

ApplicationIHC

IF5.615

SpeciesMouse,Human

PMID22343731
Foxc2 enhances proliferation and inhibits apoptosis through activating Akt/mTORC1 signaling pathway in mouse preadipocytes

JournalJournal Of Lipid Research

ApplicationWB

IF4.483

SpeciesMouse

PMID26113535
Let‐7f Inhibits Glioma Cell Proliferation, Migration, and Invasion by Targeting Periostin

JournalJournal Of Cellular Biochemistry

ApplicationWB

IF4.237

SpeciesHuman

PMID25735962