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Raf1 polyclonal antibody

货号: BS6463

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS6463

Product Name :Raf1 polyclonal antibody

Swiss-Prot :P04049

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200

Background :This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :73KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :CMD1NN;CRAF;NS5;Raf-1;c-Raf;RAF1

Immunogen :Recombinant fusion protein of human Raf1(NP_002871.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of Mouse liver, using Raf1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.

Immunohistochemistry of paraffin-embedded human colon carcinoma using Raf1 Rabbit pAb at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunofluorescence analysis of HeLa cells using Raf1 Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH-3T3 cells using Raf1 Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.

Raf kinase inhibitor protein inhibits esophageal cancer cell invasion through downregulation of matrix metalloproteinase expression

JournalOncology Reports

ApplicationWB

IF3.041

SpeciesHuman

PMID
FOXC2 promotes colorectal cancer metastasis by directly targeting MET

JournalOncogene

ApplicationWB

IF7.971

SpeciesHuman

PMID25381815
UHRF2 promotes Hepatocellular Carcinoma Progression by Upregulating ErbB3/Ras/Raf Signaling Pathway

JournalInternational Journal of Medical Sciences

ApplicationWB

IF3.738

SpeciesHuman

PMID34400880