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[KO Validated] IGF1R polyclonal antibody

货号: BS6458

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS6458

Product Name :[KO Validated] IGF1R polyclonal antibody

Swiss-Prot :P08069

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB

Application_all :WB,1:500 - 1:2000

Background :This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :100KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :IGF1R;CD221;IGFIR;IGFR;JTK13

Immunogen :Recombinant fusion protein of human IGF1R(NP_000866.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts from normal and IGF1R knockout 293T cells, using IGF1R antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 10s.

Western blot analysis of extracts of various cell lines, using IGF1R antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 10s.

Local insulin-like growth factor-I of ventral prostate was upregulated during long-term castration and may function through the autocrine system

JournalProstate Cancer And Prostatic Diseases

ApplicationIF,IHC

IF4.311

SpeciesRat

PMID21403669