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BLNK (phospho-Y84) polyclonal antibody

货号: BS64331

价格：￥ 1350

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS64331

Product Name :BLNK (phospho-Y84) polyclonal antibody

Swiss-Prot :Q8WV28

Host :Rabbit

Reactivity :Human,Rat,Mouse

Applications :WB

Application_all :WB: 1:500~1:1000

Background :Cross-linking of the B cell receptor (BCR) activates a variety of signaling pathways involved in processes such as cell proliferation and apoptosis. Intracellular protein tyrosine kinases such as Syk and Lyn have been implicated in this BCR signal transduction and are thought to play an important role in B cell development. BLNK (for B cell linker protein) is a central linker protein in B cells which has been shown to associate with the effector proteins GRB2, Vav, Nck and PLCγ following activation of the B cell receptor. The two forms of BLNK, p68 and p70, arise from alternate splicing of the human BLNK gene transcript. BLNK is phosphorylated by the Syk tyrosine kinase, which in turn permits activation of downstream effector proteins including GRB2 and PLCγ.

Product :1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.3.

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :BLNK (phospho-Y84) polyclonal antibody detects endogenous levels of BLNK protein only when phosphorylated at Try84 .

BiowMW :~ 70 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :B cell receptor signaling,

Alternative Name :B-cell linker protein; B-cell adapter containing a SH2 domain protein; B-cell adapter containing a Src homology 2 domain protein; Cytoplasmic adapter protein; Src homology 2 domain-containing leukocyte protein of 65 kDa; SLP-65; BLNK; BASH; SLP65

Immunogen :Synthetic phosphopeptide derived from human BLNK around the phosphorylation site of Tyrosine805.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of BLNK (phospho-Y84) polyclonal antibody at 1:500 dilution Lane1:PC12 whole cell lysate(40ug) Lane2:CT26 whole cell lysate(40ug) Lane3:HCT116 whole cell lysate(40ug) Lane4:Jurkat whole cell lysate(40ug) Lane5:Myla2059 whole cell lysate(40ug)