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c-Fos polyclonal antibody

货号: BS6433

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS6433

Product Name :c-Fos polyclonal antibody

Swiss-Prot :P01100

Host :Rabbit

Reactivity :Human

Applications :WB, IP

Application_all :WB,1:500 - 1:2000|IP,1:50 - 1:200

Background :The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :55-60KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :FOS;AP-1;C-FOS;p55;c-Fos

Immunogen :Recombinant fusion protein of human c-Fos(NP_005243.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of HeLa cells, using c-Fos antibody at 1:1000 dilution.HeLa cells were treated by PMA/TPA at 37℃ for 15 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.

Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug c-Fos antibody . Western blot was performed from the immunoprecipitate using c-Fos antibody at a dilution of 1:1000.

Effect of ART1 on the proliferation and migration of mouse colon carcinoma CT26 cells in vivo

JournalMolecular Medicine Reports

ApplicationWB

IF1.851

SpeciesMouse

PMID28138708
Streptolysin O derived from Streptococcus pyogenes inhibits RANKL‑induced osteoclastogenesis through the NF‑κB signaling pathway

JournalMolecular Medicine Reports

ApplicationWB

IF2.952

SpeciesMouse

PMID30431141