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FGF2 polyclonal antibody

货号: BS6432

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS6432

Product Name :FGF2 polyclonal antibody

Swiss-Prot :P09038

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200

Background :The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :26KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :BFGF;FGF-2;FGFB;HBGF-2;Basic FGF;FGF2

Immunogen :Recombinant fusion protein of human FGF2(NP_001997.5).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of 293T cells, using FGF2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit .
Exposure time: 180s.

Immunohistochemistry of paraffin-embedded Mouse kidney using FGF2 Rabbit pAb at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded Rat ovary using FGF2 Rabbit pAb at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded Human colon using FGF2 Rabbit pAb at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Oridonin inhibits tumor angiogenesis and induces vessel normalization in experimental colon cancer

JournalJournal of Cancer

ApplicationWB

IF4.207

SpeciesMouse

PMID33976735
Functional improvement of collagen-based bioscaffold to enhance periodontal-defect healing via combination with dietary antioxidant and COMP-angiopoietin 1

JournalMaterials Science & Engineering C-Materials for Biological Applications

ApplicationIHC

IF7.328

SpeciesRat

PMID
n-acetyl-l-cysteine stimulates bone healing by recovering the age-associated degenerative complications relative to osteoblastic Wntless ablation

Journal10.1016/j.biopha.2024.117761

ApplicationIHC

IF6.9

SpeciesMouse

PMID39700869