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[KO Validated] Caspase-3 polyclonal antibody

货号: BS6428

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS6428

Product Name :[KO Validated] Caspase-3 polyclonal antibody

Swiss-Prot :P42574

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC, IP

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:100|IF/ICC,1:50 - 1:100|IP,1:50 - 1:100

Background :This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :17kDa/35kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :CPP32;CPP32B;SCA-1;Active Caspase 3;CASP3;active Caspase-3;Caspase 3;Caspase-3 p12;caspase-3

Immunogen :Recombinant fusion protein of human Caspase-3(NP_004337.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using Caspase-3 Rabbit pAb at 1:1000 dilution.Jurkat cells were treated by Etoposide at 37℃ for 5 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit .
Exposure time: 3min.

Western blot analysis of extracts from normal and Caspase-3 knockout 293T cells, using Caspase-3 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 5s.

Immunohistochemistry of paraffin-embedded human mammary cancer using Caspase-3 antibody at dilution of 1:200 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] Caspase-3 Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.

Extracellular Vesicles Derived from Bone Marrow Mesenchymal Stem Cells Protect against Experimental Colitis via Attenuating Colon Inflammation, Oxidative Stress and Apoptosis

JournalPLoS One

ApplicationIHC,WB

IF2.776

SpeciesRat

PMID26469068
Mitochondrial division inhibitor 1 (Mdivi-1) offers neuroprotection through diminishing cell death and improving functional outcome in a mouse model of traumatic brain injury

JournalBrain Research

ApplicationWB

IF2.733

SpeciesMouse

PMID26596858
Downregulation of angiogenin inhibits the growth and induces apoptosis in human bladder cancer cells through regulating AKT/mTOR signaling pathway

JournalJournal Of Molecular Histology

ApplicationWB,IHC

IF2.611

SpeciesHuman,Mouse

PMID25564356
Neuroglobin Attenuates Beta Amyloid-Induced Apoptosis Through Inhibiting Caspases Activity by Activating PI3K/Akt Signaling Pathway

JournalJournal Of Molecular Neuroscience

ApplicationWB

IF2.577

SpeciesMouse,Human

PMID26346601
Apoptotic and nonapoptotic function of caspase 7 in spermatogenesis

JournalAsian Journal Of Andrology

ApplicationIHC

IF2.448

SpeciesMouse

PMID26643564