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BRCA1 polyclonal antibody

货号: BS6423

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS6423

Product Name :BRCA1 polyclonal antibody

Swiss-Prot :P38398

Host :Rabbit

Reactivity :Human

Applications :IHC

Application_all :IHC,1:50 - 1:200

Background :This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :Refer to figures

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :BRCA1;BRCAI;BRCC1;BROVCA1;FANCS;IRIS;PNCA4;PPP1R53;PSCP;RNF53

Immunogen :Recombinant fusion protein of human BRCA1(NP_009225.1).

Conjugate :Unconjugated

Modification :Unmodification

Immunohistochemistry of paraffin-embedded human breast cancer using BRCA1 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ATM–ESCO2–SMC3 axis promotes 53BP1 recruitment in response to DNA damage and safeguards genome integrity by stabilizing cohesin complex

JournalNUCLEIC ACIDS RESEARCH

ApplicationIF

IF14.9

SpeciesHuman

PMID37377435