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Bim (phospho-S69/S65) polyclonal antibody

货号: BS64039

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS64039

Product Name :Bim (phospho-S69/S65) polyclonal antibody

Swiss-Prot :O43521

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB

Application_all :WB: 1:500~1:1000

Background :Pro-apototic Bcl-2 family members promote cell death by neutralizing their anti-apoptotic relatives, which otherwise maintain cell viability by regulating caspase activity. Bim belongs to the BH3-only subgroup of Bcl-2 related proteins, and exists in three distinct isoforms, BimS (short), BimL (long) and BimEL (extra long). ERK1/2 phosphorylates BimEL, resulting in rapid degradation of the isoform via the proteasome pathway. At least three sites for ERK1/2 phosphorylation exist on BimEL, whereas ERK1/2 does not effect BimS and BimL, implying a unique role for BimEL in cell survival signaling.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -22°C long term. Avoid freeze-thaw cycles.

Specificity :p-Bim (S69/S65) polyclonal antibody detects endogenous levels of human Bim protein only when phosphorylated at Ser69. This antibody also recognizes mouse or rat Bim Protein when phosphorylated at Ser65.

BiowMW :~ 25 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Regulation of Apoptosis,Inhibition of Apoptosis,Mitochondrial Control of Apoptosis,

Alternative Name :Bcl-2-like protein 11; Bcl2-L-11; Bcl2-interacting mediator of cell death; BCL2L11; BIM

Immunogen :A synthetic peptide corresponding to residues in Human Bim around the phosphorylation site of Ser69.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-Bim (S69/S65) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:sp2/0 whole cell lysate

HCRP-1 regulates EGFR–AKT–BIM-mediated anoikis resistance and serves as a prognostic marker in human colon cancer

JournalCell Death & Disease

ApplicationWB

IF5.959

SpeciesHuman

PMID30518879