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ATM (phospho-S1987) polyclonal antibody

货号: BS64020

价格：￥ 1350

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

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No. :BS64020

Product Name :ATM (phospho-S1987) polyclonal antibody

Swiss-Prot :Q13315

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB

Application_all :WB: 1:500~1:1000

Background :The phosphatidylinositol kinase (PIK) family members fall into two distinct subgroups. The first subgroup contains proteins such as the PI 3- and PI 4-kinases and the second group comprises the PIK-related kinases. The PIK-related kinases include Atm, DNA-PKCS and FRAP. These proteins have in common a region of homology at their carboxy-termini that is not present in the PI 3- and PI 4-kinases. The Atm gene is mutated in the autosomal recessive disorder ataxia telangiectasia (AT) that is characterized by cerebellar degeneration (ataxia) and the appearance of dilated blood vessels (telangiec-tases) in the conjunctivae of the eyes. AT cells are hypersensitive to ionizing radiation, impaired in mediating the inhibition of DNA synthesis and display delays in p53 induction.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-ATM (S1987) polyclonal antibody detects endogenous levels of ATM protein only when phosphorylated at ser1987.

BiowMW :~ 370 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Cell Cycle Control G1 S Checkpoint,Cell Cycle G2 M DNA Damage Signaling Pathway,Mitochondrial Control of Apoptosis,

Alternative Name :Serine-protein kinase ATM; Ataxia telangiectasia mutated; A-T mutated; ATM

Immunogen :A synthetic peptide corresponding to residues in Human ATM around the phosphorylation site of ser1987.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-ATM (S1987) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate treated with UV(24h) Lane2:Raw264.7 whole cell lysate treated with UV(24h)

Western blot (WB) analysis of ATM (phospho-S1987) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:HEK293T treated with TNFα(20ng/ml) for 10 minutes whole cell lysate Lane3:HEK293T treated with TNFα(20ng/ml) for 1 hour whole cell lysate Lane4:HEK293T treated with TNFα(20ng/ml) for 4 hours whole cell lysate Lane5:HepG2 whole cell lysate(40ug) Lane6:HepG2 treated with UV for 5 minutes then repaired for 3 hours whole cell lysate(40ug) Lane7:HepG2 treated with UV for 5 minutes then repaired for 4 hours whole cell lysate(40ug) Lane8:HepG2 treated with UV for 5 minutes then repaired for 18 hours whole cell lysate(40ug)

Phosphorylated Hsp27 activates ATM-dependent p53 signaling and mediates the resistance of MCF-7 cells to doxorubicin-induced apoptosis

JournalCellular Signalling

ApplicationWB

IF3.388

SpeciesHuman

PMID23357534