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AGPAT1 polyclonal antibody

货号: BS6317

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS6317

Product Name :AGPAT1 polyclonal antibody

Swiss-Prot :Q99943

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IP

Application_all :WB,1:500 - 1:2000|IP,1:50 - 1:100

Background :This gene encodes an enzyme that converts lysophosphatidic acid (LPA) into phosphatidic acid (PA). LPA and PA are two phospholipids involved in signal transduction and in lipid biosynthesis in cells. This enzyme localizes to the endoplasmic reticulum. This gene is located in the class III region of the human major histocompatibility complex. Alternative splicing results in two transcript variants encoding the same protein.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :32kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :AGPAT1;1-AGPAT1;G15;LPAAT-alpha;LPAATA

Immunogen :Recombinant fusion protein of human AGPAT1(NP_006402.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using AGPAT1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.

Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug AGPAT1 antibody . Western blot was performed from the immunoprecipitate using AGPAT1 antibody at a dilution of 1:1000.