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BDKRB1 polyclonal antibody

货号: BS6300

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

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No. :BS6300

Product Name :BDKRB1 polyclonal antibody

Swiss-Prot :P46663

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :Bradykinin, a 9 aa peptide, is generated in pathophysiologic conditions such as inflammation, trauma, burns, shock, and allergy. Two types of G-protein coupled receptors have been found which bind bradykinin and mediate responses to these pathophysiologic conditions. The protein encoded by this gene is one of these receptors and is synthesized de novo following tissue injury. Receptor binding leads to an increase in the cytosolic calcium ion concentration, ultimately resulting in chronic and acute inflammatory responses. Several transcript variants encoding different isoforms have been found for this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :40kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :BDKRB1;B1BKR;B1R;BDKRB2;BKB1R;BKR1;BRADYB1

Immunogen :A synthetic peptide of human BDKRB1(NP_000701.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using BDKRB1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 5s.

Immunofluorescence analysis of A549 cells using BDKRB1 Rabbit pAb at dilution of 1:250 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of C6 cells using BDKRB1 Rabbit pAb at dilution of 1:250 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH-3T3 cells using BDKRB1 Rabbit pAb at dilution of 1:250 . Blue: DAPI for nuclear staining.