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PI3 Kinase p85 alpha polyclonal antibody

货号: BS6291

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS6291

Product Name :PI3 Kinase p85 alpha polyclonal antibody

Swiss-Prot :P27986

Host :Rabbit

Reactivity :Human, Rat

Applications :WB, IHC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200

Background :Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :85KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :AGM7;GRB1;IMD36;p85;p85-ALPHA;PI3 Kinase p85 alpha;PIK3R1

Immunogen :Recombinant fusion protein of human Kinase p85 alpha(NP_852664.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of U-87MG cells, using PI3 Kinase p85 alpha antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 180s.

Immunohistochemistry of paraffin-embedded rat brain using PI3 Kinase p85 alpha antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Persistent Transmissible Gastroenteritis Virus Infection Enhances Enterotoxigenic Escherichia coli K88 Adhesion by Promoting Epithelial-Mesenchymal Transition in Intestinal Epithelial Cells | Journal of Virology

JournalJournal Of Virology

ApplicationWB

IF5.103

SpeciesPig

PMID28794036
Farrerol Inhibits Vascular Smooth Muscle Cell Proliferation and Protects Them From Oxidative Injury via Bidirectional Modulation of the PI3K/Akt/mTOR Signaling Pathway

JournalNatural Product Communications

ApplicationWB

IF1.496

SpeciesRat

PMID