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eIF4EBP1 polyclonal antibody

货号: BS6290

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS6290

Product Name :eIF4EBP1 polyclonal antibody

Swiss-Prot :Q13541

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:1000|IHC,1:50 - 1:200|IF/ICC,1:100 - 1:200

Background :This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :15-20KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :4E-BP1;4EBP1;BP-1;PHAS-I;EIF4EBP1

Immunogen :A synthetic peptide of human eIF4EBP1(NP_004086.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using eIF4EBP1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.

Apoptosis Induction by Iron Radiation via Inhibition of Autophagy in Trp53+/-Mouse Testes: Is Chronic Restraint-Induced Stress a Modifying Factor?

JournalInternational Journal of Biological Sciences

ApplicationWB

IF4.067

SpeciesMouse

PMID29989073