产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

NRF2 polyclonal antibody

货号: BS6286

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS6286

Product Name :NRF2 polyclonal antibody

Swiss-Prot :Q16236

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200

Background :This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :97-100KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :NFE2L2;HEBP1;NRF2

Immunogen :Recombinant fusion protein of human NRF2(NP_006155.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of various lysates, using NRF2 antibody at ARC50393-02 dilution.HeLa cells were treated by MG132 at 37℃ for 90 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.

Western blot analysis of various lysates, using NRF2 antibody at ARC50393-02 dilution.NIH3T3 cells were treated by MG132 at 37℃ for 90 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.

Western blot analysis of various lysates, using NRF2 antibody at ARC50393-02 dilution.C6 cells were treated by MG132 at 37℃ for 90 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.

Immunohistochemistry of paraffin-embedded human breast cancer using NRF2 Rabbit pAb at dilution of 1:100 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

PGD2 and PGE2 regulate gene expression of Prx 6 in primary macrophages via Nrf2

JournalFree Radical Biology And Medicine

ApplicationWB

IF7.376

SpeciesMouse

PMID
Oxymatrine prevents hypoxia- and monocrotaline-induced pulmonary hypertension in rats

JournalFree Radical Biology And Medicine

ApplicationWB

IF7.376

SpeciesRat

PMID24440469
Sodium hydrosulfide alleviates lung inflammation and cell apoptosis following resuscitated hemorrhagic shock in rats

JournalActa Pharmacologica Sinica

ApplicationWB

IF6.15

SpeciesRat

PMID
Drug resistance associates with activation of Nrf2 in MCF-7/DOX cells, and wogonin reverses it by down-regulating Nrf2-mediated cellular defense response

JournalMolecular Carcinogenesis

ApplicationWB

IF4.784

SpeciesHuman

PMID
Attenuation of Cigarette Smoke-Induced Airway Mucus Production by Hydrogen-Rich Saline in Rats

JournalPLoS One

ApplicationWB

IF2.776

SpeciesRat

PMID24376700