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CD3D polyclonal antibody

货号: BS6280

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS6280

Product Name :CD3D polyclonal antibody

Swiss-Prot :P04234

Host :Rabbit

Reactivity :Human, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:100|IF/ICC,1:50 - 1:100

Background :The protein encoded by this gene is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction. The encoded membrane protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T-cells. Defects in this gene are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (SCIDBNK). Two transcript variants encoding different isoforms have been found for this gene. Other variants may also exist, but the full-length natures of their transcripts has yet to be defined.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :19kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :CD3D;CD3-DELTA;IMD19;T3D

Immunogen :Recombinant fusion protein of human CD3D(NP_000723.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of Jurkat cells, using CD3D antibody at 1:500 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat spleen using CD3D antibody at dilution of 1:200 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunofluorescence analysis of Jurkat cells using CD3D antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Expression of Heat Shock Protein 27 in Benign Prostatic Hyperplasia with Chronic Inflammation

JournalMedical Science Monitor

ApplicationIHC

IF2.649

SpeciesHuman

PMID26434601