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TDP-43/TARDB polyclonal antibody

货号: BS6221

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS6221

Product Name :TDP-43/TARDB polyclonal antibody

Swiss-Prot :Q13148

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IP, RIP

Application_all :WB,1:500 - 1:2000|IP,1:50 - 1:100|RIP,1:20 - 1:50

Background :HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :42kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :ALS10;TDP-43;TARDBP;TDP43

Immunogen :Recombinant fusion protein of human TDP-43/TARDB(NP_031401.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using TDP-43/TARDB antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Immunoprecipitation analysis of 200ug extracts of SW620 cells using 3ug TDP-43/TARDB antibody . Western blot was performed from the immunoprecipitate using TDP-43/TARDB antibody at a dilution of 1:500.