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ASC/TMS1 polyclonal antibody

货号: BS6205

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS6205

Product Name :ASC/TMS1 polyclonal antibody

Swiss-Prot :Q9ULZ3

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200

Background :This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :19KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :PYCARD;ASC;CARD5;TMS;TMS-1;TMS1;PYD and CARD domain containing;ASC / TMS1

Immunogen :A synthetic peptide of human ASC/TMS1(NP_037390.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of Mouse spleen, using ASC/TMS1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.

Western blot analysis of extracts of Rat spleen, using ASC/TMS1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 180s.

Immunohistochemistry of paraffin-embedded human colon using ASC/TMS1 Rabbit pAb at dilution of 1:150 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded human tonsil using ASC/TMS1 Rabbit pAb at dilution of 1:150 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Bone marrow mesenchymal stem cell-derived exosomes attenuate cerebral ischemia-reperfusion injury-induced neuroinflammation and pyroptosis by modulating microglia M1/M2 phenotypes

JournalExperimental Neurology

ApplicationWB

IF4.691

SpeciesRat

PMID33741350
Simiao pill inhibits epithelial mesenchymal transition in a mouse model of chronic hyperuricemic nephropathy by inhibiting NLRP3 inflammasome activation

JournalBMC Complementary Medicine and Therapies

ApplicationWB

IF2.838

SpeciesMouse

PMID36271349
Chlorogenic acid protects against myocardial ischemia–reperfusion injury in mice by inhibiting Lnc Neat1/NLRP3 inflammasome-mediated pyroptosis

JournalScientific Reports

ApplicationWB

IF4.6

SpeciesMouse

PMID37853132