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ATG16L1 polyclonal antibody

货号: BS61617

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS61617

Product Name :ATG16L1 polyclonal antibody

Swiss-Prot :Q676U5

Host :Rabbit

Reactivity :Human

Applications :WB

Application_all :WB: 1:500~1:1000

Background :Mammalian Atg16L1, containing an amino-terminal coiled coil domain and carboxyl-terminal WD-repeats, has multiple isoforms produced by alternative splicing. Atg16L1 provides a functional link between the two crucial ubiquitin-like conjugation systems of autophagy. Atg16L1 binds Atg5 of the Atg12-Atg5 conjugate forming an 800 kDa multimeric complex. The Atg12-Atg-5-Atg16L1 complex localizes to pre-autophagosomal membranes where it determines the site of LC3 lipidation and catalyzes the reaction required for the formation of mature autophagosomes. Genome-wide association scanning revealed variations in the Atg16L1 gene associated with Crohn's disease. Mice lacking the coiled coil domain of Atg16L1 have impaired autophagosome formation and elevated inflammatory cytokines, consistent with its role in inflammatory disease pathogenesis. Hypomorphic Atg16L1 mice also show defects in autophagy and abnormalities in intestinal Paneth cell function similar to that found in Crohn's disease.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :ATG16L1 polyclonal antibody detects endogenous levels of ATG16L1 protein.

BiowMW :~ 72 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Autophagy Signaling,

Alternative Name :Autophagy-related protein 16-1; APG16-like 1

Immunogen :Synthetic peptide, corresponding to amino acids 93-137 of Human ATG16L1

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of ATG16L1 polyclonal antibody at 1:500 dilution Lane1:A549 whole cell lysate(40ug) Lane2:PC3 whole cell lysate(40ug) Lane3:HCT116 whole cell lysate(40ug) Lane4:HepG2 whole cell lysate(40ug)