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SOX2 polyclonal antibody

货号: BS6161

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS6161

Product Name :SOX2 polyclonal antibody

Swiss-Prot :P48431

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC, IP

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200|IP,1:50 - 1:200

Background :This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT).

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :35KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :ANOP3;MCOPS3;SOX2;SRY-box 2

Immunogen :Recombinant fusion protein of human SOX2(NP_003097.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of MCF7 cells, using SOX2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 60s.

Western blot analysis of extracts of various cell lines, using SOX2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 180s.

Immunohistochemistry of paraffin-embedded mouse embryos using [KO Validated] SOX2 Rabbit pAb at dilution of 1:100 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunofluorescence analysis of F9 cells using [KO Validated] SOX2 Rabbit pAb at dilution of 1:50 . Blue: DAPI for nuclear staining.

Hippocampal miR-211-5p regulates neurogenesis and depression-like behaviors in the rat

JournalNeuropharmacology

ApplicationIF

IF5.25

SpeciesRat

PMID34062164
Macrophages activate mesenchymal stem cells to acquire cancer‑associated fibroblast‑like features resulting in gastric epithelial cell lesions and malignant transformation in vitro

JournalOncology Letters

ApplicationWB

IF2.311

SpeciesHuman

PMID30655826