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Ambra1 polyclonal antibody

货号: BS6138

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

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No. :BS6138

Product Name :Ambra1 polyclonal antibody

Swiss-Prot :Q9C0C7

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200

Background :Substrate-recognition component of a DCX (DDB1-CUL4-X-box E3 ubiquitin-protein ligase complex involved in cell cycle control and autophagy. The DCX(AMBRA1 complex specifically mediates the polyubiquitination of target proteins such as BECN1, CCND1, CCND2, CCND3, ELOC and ULK1. Acts as an upstream master regulator of the transition from G1 to S cell phase: AMBRA1 specifically recognizes and binds phosphorylated cyclin-D (CCND1, CCND2 and CCND3, leading to cyclin-D ubiquitination by the DCX(AMBRA1 complex and subsequent degradation. By controlling the transition from G1 to S phase and cyclin-D degradation, AMBRA1 acts as a tumor suppressor that promotes genomic integrity during DNA replication and counteracts developmental abnormalities and tumor growth.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :130-150KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :AMBRA1;DCAF3;WDR94;FLJ20294

Immunogen :Recombinant fusion protein of human Ambra1(NP_060219.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of PANC-1, using Ambra1 antibody at 1:890 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit .
Exposure time: 30s.

Immunohistochemistry of paraffin-embedded rat lung using AMBRA1 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded mouse lung using AMBRA1 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunofluorescence analysis of NIH/3T3 cells using Ambra1 Rabbit pAb at dilution of 1:50 . Blue: DAPI for nuclear staining.