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[KO Validated] Glutathione Synthetase (GSS) polyclonal antibody

货号: BS6121

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS6121

Product Name :[KO Validated] Glutathione Synthetase (GSS) polyclonal antibody

Swiss-Prot :P48637

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB,IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. The protein encoded by this gene functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in this gene are a cause of glutathione synthetase deficiency.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :52KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :GSS;GSHS;HEL-S-64p;HEL-S-88n

Immunogen :Recombinant fusion protein of human Glutathione Synthetase (Glutathione Synthetase (GSS))(NP_000169.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts from normal and Glutathione Synthetase ) knockout 293T cells, using Glutathione Synthetase ) antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 5s.

Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] Glutathione Synthetase Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using [KO Validated] Glutathione Synthetase Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U2OS cells using [KO Validated] Glutathione Synthetase Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.