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Arp3 polyclonal antibody

货号: BS6115

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS6115

Product Name :Arp3 polyclonal antibody

Swiss-Prot :P61158

Host :Rabbit

Reactivity :Human, Mouse, Rat, Monkey

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:10 - 1:100

Background :The specific function of this gene has not yet been determined; however, the protein it encodes is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusion. Three transcript variants encoding two different isoforms have been found for this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :47kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :ACTR3;ARP3

Immunogen :Recombinant fusion protein of human Arp3(NP_005712.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using Arp3 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .

Immunofluorescence analysis of HeLa cells using Arp3 antibody . Blue: DAPI for nuclear staining.