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14-3-3 epsilon polyclonal antibody

货号: BS6109

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS6109

Product Name :14-3-3 epsilon polyclonal antibody

Swiss-Prot :P62258

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :29-32Kda

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :YWHAE;14-3-3E;HEL2;KCIP-1;MDCR;MDS

Immunogen :Recombinant fusion protein of human 14-3-3 epsilon(NP_006752.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using 14-3-3 epsilon antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.

Immunofluorescence analysis of H9C2 cells using 14-3-3 epsilon antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of L929 cells using 14-3-3 epsilon antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U2OS cells using 14-3-3 epsilon antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Helicobacter pylori infection combined with DENA revealed altered expression of p53 and 14-3-3 isoforms in Gulo−/− mice

JournalChemico-Biological Interactions

ApplicationIHC

IF3.723

SpeciesMouse

PMID
Proteomic Analysis of Cellular Response Induced by Multi-Walled Carbon Nanotubes Exposure in A549 Cells

JournalPLoS One

ApplicationWB

IF2.74

SpeciesHuman

PMID
Proteomic analysis of differentially expressed proteins in vitamin C-treated AGS cells

JournalBmc Biochemistry

ApplicationWB

IF1.75

SpeciesHuman

PMID
Proteomic analysis of selective cytotoxic anticancer properties of flavonoids isolated from Citrus platymamma on A549 human lung cancer cells

JournalMolecular Medicine Reports

ApplicationWB

IF2.1

SpeciesHuman

PMID27573346