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TLR7 polyclonal antibody

货号: BS6095

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

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No. :BS6095

Product Name :TLR7 polyclonal antibody

Swiss-Prot :Q9NYK1

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is predominantly expressed in lung, placenta, and spleen, and lies in close proximity to another family member, TLR8, on chromosome X.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :150KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :TLR7;TLR7-like

Immunogen :Recombinant fusion protein of human TLR7(NP_057646.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of Mouse liver, using TLR7 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.

Immunofluorescence analysis of mouse spleen cells using TLR7 Rabbit pAb at dilution of 1:25 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of rat spleen cells using TLR7 Rabbit pAb at dilution of 1:25 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of THP-1 cells using TLR7 Rabbit pAb at dilution of 1:25 . Blue: DAPI for nuclear staining.