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[KO Validated] DJ-1/PARK7 polyclonal antibody

货号: BS6091

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS6091

Product Name :[KO Validated] DJ-1/PARK7 polyclonal antibody

Swiss-Prot :Q99497

Host :Rabbit

Reactivity :Human, Mouse

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :The product of this gene belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. Defects in this gene are the cause of autosomal recessive early-onset Parkinson disease 7. Two transcript variants encoding the same protein have been identified for this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :20kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :DJ-1;DJ1;GATD2;HEL-S-67p;PARK7

Immunogen :Recombinant fusion protein of human DJ-1/PARK7(NP_001116849.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using DJ-1/PARK7 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Western blot analysis of extracts from normal and DJ-1/PARK7 knockout 293T cells, using DJ-1/PARK7 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 3s.

Immunofluorescence analysis of HeLa cells using DJ-1/PARK7 antibody . Blue: DAPI for nuclear staining.