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eEF1A1 polyclonal antibody

货号: BS6077

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

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No. :BS6077

Product Name :eEF1A1 polyclonal antibody

Swiss-Prot :P68104

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:20 - 1:100

Background :This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :50kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :EEF1A1;CCS-3;CCS3;EE1A1;EEF-1;EEF1A;EF-Tu;EF1A;GRAF-1EF;HNGC:16303;LENG7;PTI1;eEF1A-1

Immunogen :Recombinant fusion protein of human eEF1A1(NP_001393.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using eEF1A1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 10s.

Immunofluorescence analysis of U2OS cells using eEF1A1 Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.

Mycobacterium tuberculosis Mce2E suppresses the macrophage innate immune response and promotes epithelial cell proliferation

JournalCellular & Molecular Immunology

ApplicationWB

IF11.53

SpeciesMouse

PMID29572547
CircRNA_0050463 promotes influenza A virus replication by sponging miR-33b-5p to regulate EEF1A1

JournalVeterinary Microbiology

ApplicationWB

IF3.293

SpeciesHuman

PMID33517193