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ATP6V0A2 polyclonal antibody

货号: BS60737

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS60737

Product Name :ATP6V0A2 polyclonal antibody

Swiss-Prot :Q9Y487

Host :Rabbit

Reactivity :Human,Mouse

Applications :WB

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:207

Background :Vacuolar-type H+-ATPase (V-ATPase) is a multisubunit enzyme responsible for the acidification of eukaryotic intracellular organelles. V-ATPases pump protons against an electrochemical gradient, while F-ATPases reverse the process, thereby synthesizing ATP. A peripheral V1 domain, which is responsible for ATP hydrolysis, and an integral V0 domain, which is responsible for proton translocation, comprise the V-ATPase complex. Nine subunits (A–H) make up the V1 domain and five subunits (A, D, C, C' and C") make up the V0 domain. As part of the V0 domain, V-ATPase A2 (ATPase, H+ transporting, lysosomal V0 subunit a2), consists of 856 amino acids and is also known as ATP6V0A2, V-type proton ATPase subunit a isoform 2, vacuolar proton translocating ATPase subunit a isoform 2, lysosomal H(+)-transporting ATPase V0 subunit a2 or TJ6. V-ATPase A2 is a multi-pass membrane protein with localization in the cell membrane, endosome membrane and the subapical vesicles of the kidney’s proximal tubules. V-ATPase A2 plays an important role in Golgi function by regulating pH. Wrinkly skin syndrome (WSS) and cutis laxa type II (ARCL type II) are caused as a result of V-ATPase A2 defects.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 102% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -27°C long term. Avoid freeze-thaw cycles.

Specificity :ATP6V0A2 polyclonal antibody detects endogenous levels of ATP6V0A2 protein.

BiowMW :~ 98 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :V-type proton ATPase 116 kDa subunit a isoform 2; V-ATPase 116 kDa isoform a2; Lysosomal H(+)-transporting ATPase V0 subunit a2 TJ6; Vacuolar proton translocating ATPase 116 kDa subunit a isoform 2; ATP6V0A2

Immunogen :A synthetic peptide corresponding to residues in Human ATP6V0A2.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of ATP6V0A2 polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:sp2/0 whole cell lysate Lane3:H9C9 whole cell lysate