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Desmin polyclonal antibody

货号: BS6064

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS6064

Product Name :Desmin polyclonal antibody

Swiss-Prot :P17661

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:100|IF/ICC,1:50 - 1:100

Background :This gene encodes a muscle-specific class III intermediate filament. Homopolymers of this protein form a stable intracytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane. Mutations in this gene are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :55KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :DES;CSM1;CSM2;LGMD2R;desmin;Desmin

Immunogen :Recombinant fusion protein of human Desmin(NP_001918.3).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of C2C12 cells, using Desmin antibody at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.

Immunohistochemistry of paraffin-embedded human colon using Desminmin Rabbit pAb at dilution of 1:100 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded mouse lung using Desminmin Rabbit pAb at dilution of 1:100 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunofluorescence analysis of rat heart using Desminmin Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.

Effects of matrix metalloproteinase-1 on the myogenic differentiation of bone marrow-derived mesenchymal stem cells in vitro

JournalBiochemical And Biophysical Research Communications

ApplicationWB

IF2.985

SpeciesMouse

PMID23085232
The role of the miR-31/FIH1 pathway in TGF-β-induced liver fibrosis

JournalClinical Science

ApplicationIF

IF5.223

SpeciesRat,Mouse

PMID25728779
Small activating RNA induces myogenic differentiation of rat adipose-derived stem cells by upregulating MyoD

JournalInternational Braz J Urol

ApplicationICC

IF1.046

SpeciesRat

PMID26401871