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BMP7 polyclonal antibody

货号: BS6062

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

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No. :BS6062

Product Name :BMP7 polyclonal antibody

Swiss-Prot :P18075

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200

Background :This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer, which plays a role in bone, kidney and brown adipose tissue development. Additionally, this protein induces ectopic bone formation and may promote fracture healing in human patients.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :49KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :BMP7;OP-1

Immunogen :Recombinant fusion protein of human BMP7(NP_001710.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using BMP7 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.

Immunohistochemistry of paraffin-embedded mouse kidney using BMP7 Rabbit pAb at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunofluorescence analysis of rat kidney cells using BMP7 Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of mouse kidney cells using BMP7 Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.