产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

MMP7 polyclonal antibody

货号: BS6059

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS6059

Product Name :MMP7 polyclonal antibody

Swiss-Prot :P09237

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200

Background :This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This secreted protease breaks down proteoglycans, fibronectin, elastin and casein and differs from most MMP family members in that it lacks a conserved C-terminal hemopexin domain. The enzyme is involved in wound healing, and studies in mice suggest that it regulates the activity of defensins in intestinal mucosa. The gene is part of a cluster of MMP genes on chromosome 11. This gene exhibits elevated expression levels in multiple human cancers.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :20-22KDa/28KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :MMP7;MMP-7;MPSL1;PUMP-1

Immunogen :A synthetic peptide of human MMP7(NP_002414.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using MMP7 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 10s.

Western blot analysis of extracts of Rat uterus, using MMP7 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 180s.

Immunohistochemistry of paraffin-embedded human lung cancer using MMP7 Rabbit pAb at dilution of 1:150 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunofluorescence analysis of A-549 cells using MMP7 Rabbit pAb at dilution of 1:350 . Blue: DAPI for nuclear staining.

Silencing NKD2 by Promoter Region Hypermethylation Promotes Esophageal Cancer Progression by Activating Wnt Signaling

JournalJournal of Thoracic Oncology

ApplicationWB

IF12.46

SpeciesHuman

PMID27374455
Downregulation of CDK-8 inhibits colon cancer hepatic metastasis by regulating Wnt/β-catenin pathway

JournalBiomedicine & Pharmacotherapy

ApplicationWB

IF6.529

SpeciesMouse

PMID26349978
Methylation of TMEM176A is an independent prognostic marker and is involved in human colorectal cancer development

JournalEpigenetics

ApplicationWB

IF4.251

SpeciesHuman

PMID28678648
Inhibitory effect of Ginkgol C17:1 on the biological behavior of tumor cells

JournalOncology Letters

ApplicationWB

IF2.967

SpeciesHuman

PMID28454337
Type II cGMP‑dependent protein kinase inhibits the migration, invasion and proliferation of several types of human cancer cells

JournalMolecular Medicine Reports

ApplicationWB

IF1.851

SpeciesHuman

PMID28849123