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MDK polyclonal antibody

货号: BS6038

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS6038

Product Name :MDK polyclonal antibody

Swiss-Prot :P21741

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :This gene encodes a member of a small family of secreted growth factors that binds heparin and responds to retinoic acid. The encoded protein promotes cell growth, migration, and angiogenesis, in particular during tumorigenesis. This gene has been targeted as a therapeutic for a variety of different disorders. Alternatively spliced transcript variants encoding multiple isoforms have been observed.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :16KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :MDK;ARAP;MK;NEGF2;midkine

Immunogen :Recombinant fusion protein of human MDK(NP_002382.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of SH-SY5Y cells, using MDK antibody at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit .
Exposure time: 180s.

Immunofluorescence analysis of HeLa cells using MDK Rabbit pAb at dilution of 1:25 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using MDK Rabbit pAb at dilution of 1:25 . Blue: DAPI for nuclear staining.

Protease nexin-1 deficiency increases mouse hindlimb neovascularisation following ischemia and accelerates femoral artery perfusion

JournalScientific Reports

ApplicationIHC

IF4.379

SpeciesMouse

PMID34183729
Immune Escape between Endoplasmic Reticulum Stress-Related Cancer Cells and Exhausted CD8+T Cells Leads to Neoadjuvant Chemotherapy Resistance in Ovarian Cancer

JournalBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

ApplicationIF

IF2.5

SpeciesHuman

PMID39278093