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GATA2 polyclonal antibody

货号: BS6017

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS6017

Product Name :GATA2 polyclonal antibody

Swiss-Prot :P23769

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200

Background :This gene encodes a member of the GATA family of zinc-finger transcription factors that are named for the consensus nucleotide sequence they bind in the promoter regions of target genes. The encoded protein plays an essential role in regulating transcription of genes involved in the development and proliferation of hematopoietic and endocrine cell lineages. Alternative splicing results in multiple transcript variants.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :51KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :GATA2;DCML;IMD21;MONOMAC;NFE1B

Immunogen :Recombinant fusion protein of human GATA2(NP_116027.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of HeLa, using GATA2 antibody at 1:590 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 180s.

Immunohistochemistry of paraffin-embedded mouse brain using GATA2 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded rat brain using GATA2 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

NR4A1 retards adipocyte differentiation or maturation via enhancing GATA2 and p53 expression

JournalJournal Of Cellular And Molecular Medicine

ApplicationWB

IF4.658

SpeciesMouse

PMID30044048