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Bmi1 polyclonal antibody

货号: BS6015

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS6015

Product Name :Bmi1 polyclonal antibody

Swiss-Prot :P35226

Host :Rabbit

Reactivity :Human

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :This gene encodes a ring finger protein that is major component of the polycomb group complex 1 (PRC1). This complex functions through chromatin remodeling as an essential epigenetic repressor of multiple regulatory genes involved in embryonic development and self-renewal in somatic stem cells. This protein also plays a central role in DNA damage repair. This gene is an oncogene and aberrant expression is associated with numerous cancers and is associated with resistance to certain chemotherapies. A pseudogene of this gene is found on chromosome X. Read-through transcription also exists between this gene and the upstream COMM domain containing 3 (COMMD3) gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :41kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :BMI1;FLVI2/BMI1;PCGF4;RNF51;flvi-2/bmi-1;Bmi1

Immunogen :Recombinant fusion protein of human Bmi1(NP_005171.4).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of U-937 cells, using Bmi1 antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 30s.

Immunofluorescence analysis of HeLa cells using Bmi1 antibody . Blue: DAPI for nuclear staining.

H. pylori infection-induced MSC differentiation into CAFs promotes epithelial-mesenchymal transition in gastric epithelial cells

JournalInternational Journal Of Molecular Medicine

ApplicationWB

IF4.101

SpeciesHuman

PMID
Macrophages activate mesenchymal stem cells to acquire cancer‑associated fibroblast‑like features resulting in gastric epithelial cell lesions and malignant transformation in vitro

JournalOncology Letters

ApplicationWB

IF2.311

SpeciesHuman

PMID30655826