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GCLC polyclonal antibody

货号: BS6008

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS6008

Product Name :GCLC polyclonal antibody

Swiss-Prot :P48506

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:3000|IF/ICC,1:50 - 1:200

Background :Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :72kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :GCLC;GCL;GCS;GLCL;GLCLC

Immunogen :Recombinant fusion protein of human GCLC(NP_001489.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using GCLC antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 3s.

Immunofluorescence analysis of L929 cells using GCLC Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.

Hepatocyte-Specific HuR Protects Against Acetaminophen-Induced Liver Injury in Mice

Journal10.1111/jcmm.70246

ApplicationWB

IF4.3

SpeciesMouse

PMID39614424