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ATP6V1B1 polyclonal antibody

货号: BS5976

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS5976

Product Name :ATP6V1B1 polyclonal antibody

Swiss-Prot :P15313

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :Vacuolar-type H+-ATPase (V-ATPase) is a multisubunit enzyme responsible for acidification of eukaryotic intracellular organelles. V-ATPases pump protons against an electrochemical gradient, while F-ATPases reverse the process, thereby synthesizing ATP. A peripheral V1 domain, which is responsible for ATP hydrolysis, and a integral V0 domain, which is responsible for proton translocation, compose V-ATPase. Nine subunits (A–H) make up the V1 domain and five subunits (a, d, c, c' and c") make up the V0 domain. Like F-ATPase, V-ATPase most likely operates through a rotary mechanism. The V-ATPase V1 B subunit exists as two isoforms. In the inner ear, the V-ATPase B1 isoform functions in proton secretion and is required to maintain proper endolymph pH and normal auditory function. The gene encoding the human V-ATPase B1 isoform maps to chromosome 2cen-q13. Mutations in this gene cause distal renal tubular acidosis associated with sensorineural deafness. The V-ATPase B2 isoform is expressed in kidney and is the only B isoform expressed in osteoclasts.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :ATP6V1B1 polyclonal antibody detects endogenous levels of ATP6V1B1 protein.

BiowMW :~ 58 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :V-type proton ATPase subunit B, kidney isoform; V-ATPase subunit B 1; Endomembrane proton pump 58 kDa subunit; Vacuolar proton pump subunit B 1;ATP6B1; VATB; VPP3; V-ATPase B1

Immunogen :Synthetic peptide, corresponding to amino acids 426-471 of Human ATP6V1B1.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of ATP6V1B1 polyclonal antibody at 1:500 dilution Lane1:HEK293T cell lysate Lane2:Raw264.7 cell lysate Lane3:PC12 cell lysate