产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

TFEB polyclonal antibody（KU）

货号: BS5963

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS5963

Product Name :TFEB polyclonal antibody（KU）

Swiss-Prot :P19484

Host :Rabbit

Reactivity :Human,Rat,Mouse

Applications :WB

Application_all :WB: 1:500~1:1000

Background :Transcription factor EB (TFEB) is a member of the Myc-related, bHLH leucine-zipper family of transcription factors that drives the expression of a network of genes known as the Coordinated Lysosomal Expression and Regulation (CLEAR) network. TFEB specifically recognizes and binds regulatory sequences within the CLEAR box (GTCACGTGAC) of lysosomal and autophagy genes, resulting in the upregulated expression of genes involved in lysosome biogenesis and function, and regulation of autophagy. TFEB is activated in response to nutrient deprivation, stimulating translocation to the nucleus where it forms homo- or heterooligomers with other members of the microphthalmia transcription factor (MiTF) subfamily and resulting in upregulation of autophagosomes and lysosomes. Recently, it has been shown that TFEB is a component of mammalian target of rapamycin (mTOR) complex 1 (mTORC1), which regulates the phosphorylation and nuclear translocation of TFEB in response to cellular starvation and stress. During normal growth conditions, TFEB is phosphorylated at Ser211 in an mTORC1-dependent manner. Phosphorylation promotes association of TFEB with 14-3-3 family proteins and retention in the cytosol. Inhibition of mTORC1 results in a loss of TFEB phosphorylation, dissociation of the TFEB/14-3-3 complex, and rapid transport of TFEB to the nucleus where it increases transcription of CLEAR and autophagy genes. TFEB has also been shown to be activated in a nutrient-dependent manner by p42 MAP kinase (Erk2). TFEB is phosphorylated at Ser142 by Erk2 in response to nutrient deprivation, resulting in nuclear localization and activation, and indicating that pathways other than mTOR contribute to nutrient sensing via TFEB.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :TFEB polyclonal antibody detects endogenous levels of TFEB protein.

BiowMW :~ 55 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Autophagy Signaling,

Alternative Name :Transcription factor EB; Class E basic helix-loop-helix protein 35; bHLHe35; TFEB; BHLHE35

Immunogen :Synthetic peptide, corresponding to Human TFEB.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of TFEB polyclonal antibody at 1:500 dilution Lane1:Hela whole cell lysate(40ug) Lane2:SGC7901 whole cell lysate(40ug) Lane3:SHSY5Y whole cell lysate(40ug) Lane4:The Brain tissue lysate of Mouse(40ug) Lane5:C6 whole cell lysate(40ug) Lane6:HEK293T whole cell lysate(40ug)