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PCNA polyclonal antibody

货号: BS5842

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS5842

Product Name :PCNA polyclonal antibody

Swiss-Prot :P12004

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC ICC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 ICC: 1:50~1:200

Background :The proliferating cell nuclear antigen (PCNA), a protein synthesized in early G1 and S phases of the cell cycle, functions in cell cycle progression, DNA replication, and DNA repair. In early S phase, PCNA exhibits granular distribution and is absent from the nucleoli, however, in late S phase, it relocates to the nucleoli. PCNA exists in two basic forms, one involved in ongoing DNA replication. which localizes specifically to the nucleus, and a second, soluble form, not implicated in constant synthesis. Interestingly, the latter form degrades in the presence of organic solvents, rendering it undetectable by histological methods in tissues using organic fixatives, and thus also providing a method of visualizing only the synthesizing form.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :PCNA polyclonal antibody detects endogenous levels of PCNA protein.

BiowMW :~ 29, 36 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Proliferating cell nuclear antigen; Cyclin

Immunogen :Synthetic peptide, corresponding to amino acids 98-143 of Human PCNA.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of PCNA polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate Lane2:sp2/0 cell lysate Lane3:H9C2 cell lysate

Immunohistochemistry (IHC) analyzes of PCNA pAb in paraffin-embedded human colon carcinoma tissue at 1:50.showing nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Immunohistochemistry (IHC) analyzes of PCNA pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.showing nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Parental betaine supplementation promotes gosling growth with epigenetic modulation of IGF gene family in the liver

JournalJOURNAL OF ANIMAL SCIENCE

ApplicationWB

IF3.3

SpeciesGeese

PMID38483185
In ovo betaine injection improves breast muscle growth in newly hatched goslings through FXR/IGF-2 pathway

JournalPOULTRY SCIENCE

ApplicationWB,IF

IF3.8

SpeciesGoose

PMID39094501