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AKAP8 polyclonal antibody

货号: BS5602

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS5602

Product Name :AKAP8 polyclonal antibody

Swiss-Prot :O43823

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC ICC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 ICC: 1:50~1:200

Background :The type II cAMP-protein kinase (PKA) is a multifunctional kinase with a broad range of substrates. Specificity of PKA signaling is thought to be mediated by the compartmentalization of the kinase to specific sites within the cell. To maintain this specific localization, the R subunit (RII) of PKA interacts with specific RII-anchoring proteins. The family of RII-anchoring proteins has been designated A-kinase anchoring proteins (AKAP). AKAP 95, also known as AKAP 8, is a nuclear matrix protein predominantly expressed in liver, heart, pancreas, kidney and skeletal muscle. During mitosis, AKAP 95 is recruited to the chromosomes and plays an essential role in mitotic progression. Characteristic of its family, AKAP 95 participates in PKA signaling through an interaction with the RII regulatory subunit. In addition, AKAP 95 forms a complex with HA95 and HDAC3 and is required for the deacetylation of Histone H3 in mitosis.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :AKAP8 polyclonal antibody detects endogenous levels of AKAP8 protein.

BiowMW :~ 95 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :A-kinase anchor protein 8; AKAP-8; A-kinase anchor protein 95 kDa; AKAP 95; AKAP 8; AKAP95; AKAP-95;

Immunogen :Synthetic peptide, corresponding to amino acids 373-418 of Human AKAP8.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of AKAP8 polyclonal antibody at 1:500 dilution Lane1:HepG2 cell lysate Lane2:Mouse kidney tissue lysate Lane3:Rat kidney tissue lysate

Immunohistochemistry (IHC) analyzes of AKAP8 pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.showing nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.