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Stat1 (phospho-S727) polyclonal antibody

货号: BS5036

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS5036

Product Name :Stat1 (phospho-S727) polyclonal antibody

Swiss-Prot :P42224

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :Signal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-Stat1 (S727) polyclonal antibody detects endogenous levels of Stat1 protein only when phosphorylated at Ser727.

BiowMW :~ 87 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Signal transducer and activator of transcription 1-alpha/beta; Transcription factor ISGF-3 components p91/p84; STAT1; STAT1α; STAT1β

Immunogen :Synthetic phosphopeptide derived from human Stat1 around the phosphorylation site of Serine 727.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of Stat1 (phospho-S727) polyclonal antibody at 1:500 dillution Lane1:NIH-3T3 whole cell lysate(40μg) Lane2:NIH-3T3 treated with UV(4h) whole cell lysate(40μg) Lane3:NIH-3T3 treated with PMA(60ng/ml, 30min) whole cell lysate(40μg) Lane4:Hela whole cell lysate(40μg) Lane5:Hela treated with IFN-γ(100ng/ml, 15min) whole cell lysate(40μg) Lane6:Hela treated with PMA(60ng/ml, 30min) whole cell lysate(40μg)

Immunohistochemistry (IHC) analyzes of p-Stat1 (S727) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.