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PKC δ (phospho-Y52) polyclonal antibody

货号: BS4861

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS4861

Product Name :PKC δ (phospho-Y52) polyclonal antibody

Swiss-Prot :Q05655

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :Protein Kinase C delta (PKC delta) is a 78 kDa member of the novel group (nPKCs: sensitive to diacylglycerol, phosphatidylserine, and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC delta is an ubiquitously expressed PKC isozyme that has been implicated in the regulation of multiple cellular processes including cell cycle progression and apoptosis. Autophosphorylation of serine 664 (serine 662 in mouse and rat) contributes to PKC delta activity. Serum dependent phosphorylation of serine 664 is mediated by mTOR pathway, is protected from dephosphorylation by phosphorylated threonine 505 in the activation loop, and is implicated in prolactin signaling.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 97% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-PKC δ (Y52) polyclonal antibody detects endogenous levels of PKC δ protein only when phosphorylated at Tyr52.

BiowMW :~ 80 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Protein kinase C delta type; Tyrosine-protein kinase PRKCD; nPKC-delta; Sphingosine-dependent protein kinase-1; SDK1; PRKCD; PKCδ

Immunogen :Synthetic phosphopeptide derived from human PKC δ around the phosphorylation site of Tyrosine 52.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-PKC δ (Y52) polyclonal antibody at 1:500 dilution Lane1:MCF-7 cell lysate treated with serum starvation(24h) Lane2:Raw264.7 cell lysate treated with serum starvation(24h) Lane3:H9C2 cell lysate treated with serum starvation(24h)

Immunohistochemistry (IHC) analyzes of p-PKC δ (Y52) pAb in paraffin-embedded human liver carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.