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PFKFB2 (phospho-S483) polyclonal antibody

货号: BS4859

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS4859

Product Name :PFKFB2 (phospho-S483) polyclonal antibody

Swiss-Prot :O60825

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :PFK-1 undergoes activation in the presence of elevated AMP. The most potent activator is fructose-2,6-bisphosphate, which is produced by PFK-2 from the same substrate, fructose 6-phosphate. PFK-2 is bifunctional and a key regulator for PFK-1. PFK-2 catalyzes the synthesis of fructose-2,6-bisphosphate, and contains fructose-2,6-biphosphatase activity that catalyzes the degradation of fructose-2,6-bisphosphate. PFK-2 is dimeric and isoenzymes include PFK-2 liver (PFKFB1, PFRX), PFK-2 cardiac (PFKFB2), PFK-2 placental (PFKFB3, inducible PFK-2) and PFK-2 testis (PFKFB4).

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-PFKFB2 (S483) polyclonal antibody detects endogenous levels of PFKFB2 protein only when phosphorylated at Ser483.

BiowMW :~ 58 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :PFK-2 car; PFK-2 cardiac; 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2; 6PF-2-K/Fru-2,6-P2ase 2; PFK/FBPase 2; 6PF-2-K/Fru-2,6-P2ase heart-type isozyme; 6-phosphofructo-2-kinase; Fructose-2,6-bisphosphatase; PFKFB2

Immunogen :Synthetic phosphopeptide derived from human PFKFB2 around the phosphorylation site of Serine 483.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-PFKFB2 (S483) polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate treated with H2O2(100µM,30mins) Lane2:sp2/0 cell lysate treated with H2O2(100µM,30mins) Lane3:H9C2 cell lysate treated with H2O2(100µM,30mins)

Immunohistochemistry (IHC) analyzes of p-PFKFB2 (S483) pAb in paraffin-embedded human kidney carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.