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p130 Cas (phospho-Y249) polyclonal antibody

货号: BS4849

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS4849

Product Name :p130 Cas (phospho-Y249) polyclonal antibody

Swiss-Prot :P56945

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC ICC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 ICC: 1:50~1:200

Background :The p130Cas (Cas for Crk-associated substrate) is a common cellular target of phosphorylation signal via v-Crk and v-Src oncoproteins. p130Cas has a unique structure that contains a Src homology (SH)-3 domain followed by multiple YXXP motifs and a proline-rich regionp130Cas is implicated in a variety of biological processes including cell adhesion, cell migration, growth factor stimulation, cytokine receptor engagement and bacterial infection. Physiological functions of p130Cas include cardiovascular development, actin filament assembly and Src-induced cell transformation.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-p130 Cas (Y410) polyclonal antibody detects endogenous levels of p130 Cas protein only when phosphorylated at Tyr410.

BiowMW :~ 130 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Breast cancer anti-estrogen resistance protein 1; CRK-associated substrate; Cas scaffolding protein family member 1; p130cas; BCAR1; CAS; CASS1; CRKAS

Immunogen :Synthetic phosphopeptide derived from human p130 Cas around the phosphorylation site of Tyrosine 249.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-p130 Cas (Y249) polyclonal antibody at 1:500 dilution Lane1:HEK293T cell lysate treated with EGF(0.1ng/ML,30mins) Lane2:Raw264.7 cell lysate treated with EGF(0.1ng/ML,30mins) Lane3:PC12 cell lysate treated with EGF(0.1ng/ML,30mins)

Immunohistochemistry (IHC) analyzes of p-p130 Cas (Y249) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing cytoplasmic staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.