Product Name :BRCA1 (phospho-S1423) polyclonal antibody
Application_all :IHC: 1:50~1:200
Background :BRCA1 (breast and ovarian cancer susceptibility protein 1) is a nuclear phosphoprotein that plays a role in maintaining genomic stability and acts as a tumor suppressor. It combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as BASC (for BRCA1 associated genome surveillance complex). BRCA1 associates with RNA polymerase II, and through the C terminal domain, also interacts with histone deacetylase complex. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in the BRCA1 gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene.
Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :p-BRCA1 (S1423) polyclonal antibody detects endogenous levels of BRCA1 protein only when phosphorylated at Ser1423.
Note :For research use only, not for use in diagnostic procedure.
Pathway :Cell Cycle G2 M DNA Damage Signaling Pathway,
Alternative Name :Breast cancer type 1 susceptibility protein; RING finger protein 53; BRCA1; RNF53
Immunogen :Synthetic phosphopeptide derived from human BRCA1 around the phosphorylation site of Serine 1423.
Modification :Phosphorylation
