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Gemin 1 Recombinant Rabbit mAb (KU)

货号: BS46757

价格：￥ 1200

货期：现货（1-2个工作日）

25ul 50ul 100ul

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No. :BS46757

Product Name :Gemin 1 Recombinant Rabbit mAb (KU)

Swiss-Prot :Q16637

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB,IHC,IP

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:100|IP,1:50 - 1:200

Background :This gene is part of a 500 kb inverted duplication on chromosome 5q13. This duplicated region contains at least four genes and repetitive elements which make it prone to rearrangements and deletions. The repetitiveness and complexity of the sequence have also caused difficulty in determining the organization of this genomic region. The telomeric and centromeric copies of this gene are nearly identical and encode the same protein. However, mutations in this gene, the telomeric copy, are associated with spinal muscular atrophy; mutations in the centromeric copy do not lead to disease. The centromeric copy may be a modifier of disease caused by mutation in the telomeric copy. The critical sequence difference between the two genes is a single nucleotide in exon 7, which is thought to be an exon splice enhancer. Note that the nine exons of both the telomeric and centromeric copies are designated historically as exon 1, 2a, 2b, and 3-8. It is thought that gene conversion events may involve the two genes, leading to varying copy numbers of each gene. The protein encoded by this gene localizes to both the cytoplasm and the nucleus. Within the nucleus, the protein localizes to subnuclear bodies called gems which are found near coiled bodies containing high concentrations of small ribonucleoproteins (snRNPs). This protein forms heteromeric complexes with proteins such as SIP1 and GEMIN4, and also interacts with several proteins known to be involved in the biogenesis of snRNPs, such as hnRNP U protein and the small nucleolar RNA binding protein. Multiple transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2014]

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :Refer to figures

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :STIM1; D11S4896E; GOK; IMD10; STRMK; TAM; TAM1; stromal interaction molecule 1

Immunogen :Recombinant fusion protein of human STIM1(NP_003147.2).

Conjugate :Unconjugated

Modification :Unmodification

Immunohistochemistry of paraffin-embedded mouse brain using STIM1 Rabbit pAb at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug STIM1 antibody . Western blot was performed from the immunoprecipitate using STIM1 antibody at a dilution of 1:1000.