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ERK1/2 (phospho-Y204) polyclonal antibody（KU）

货号: BS4621

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS4621

Product Name :ERK1/2 (phospho-Y204) polyclonal antibody（KU）

Swiss-Prot :P27361/P28482

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively. ERK proteins are regulated by dual phosphorylation at Tyrosine 204 and 187 and Threonine 177 and 160 residues mapping within a characteristic Thr-Glu-Tyr motif. Phosphorylation at both the Threonine 202 and Tyrosine 204 residues of ERK 1 and Threonine 185 and Tyrosine 187 residues of ERK 2 is required for full enzymatic activation. The structural consequences of dual phosphorylation in ERK 2 include active site closure, alignment of key catalytic residues that interact with ATP, and remodeling of the activation loop. In response to activation, MAP kinases phosphorylate downstream components on serine and threonine. Upstream MAP kinase regulators include MAP kinase kinase (MEK), MEK kinase and Raf-1. The ERK family has three additional members: ERK 3, ERK 5 and ERK 6.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-ERK1/2 (Y204) polyclonal antibody detects endogenous levels of ERK1 protein when phosphorylated at Tyr204, and ERK2 protein when phosphorylated at Tyr187.

BiowMW :~ 42,44 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Mitogen-activated protein kinase 3; MAP kinase 3; MAPK 3; ERT2; Extracellular signal-regulated kinase 1; ERK-1; Insulin-stimulated MAP2 kinase; MAP kinase isoform p44; p44-MAPK; Microtubule-associated protein 2 kinase; p44-ERK1; MAPK3; ERK1; PRKM3; Mitogen-activated protein kinase 1; MAP kinase 1; MAPK 1; ERT1; Extracellular signal-regulated kinase 2; ERK-2; MAP kinase isoform p42; p42-MAPK; Mitogen-activated protein kinase 2; MAP kinase 2; MAPK 2; MAPK1; ERK2; PRKM1; PRKM2

Immunogen :Synthetic phosphopeptide derived from human ERK1/2 around the phosphorylation site of Tyrosine 204.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-ERK1/2 (Y204) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate treated with PMA(100nM,15mins) Lane2:sp2/0 whole cell lysate treated with PMA(100nM,15mins) Lane3:PC12 whole cell lysate treated with PMA(100nM,15mins)

Immunohistochemistry (IHC) analyzes of p-ERK1/2 (Y204) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Western blot (WB) analysis of p-ERK1/2 (Y204) pAb at 1:500 dilution Lane1:L02 starved for 16 hours whole cell lysate(40ug) Lane2:L02 starved for 16 hours then treated with LPS(1000ng/ml) for 15 minutes whole cell lysate Lane3:L02 starved for 16 hours then treated with LPS(1000ng/ml) for 30 minutes whole cell lysate

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