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IDH1 Recombinant Rabbit mAb (KU)

货号: BS45382

价格：￥ 1200

货期：现货（1-2个工作日）

25ul 50ul 100ul

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS45382

Product Name :IDH1 Recombinant Rabbit mAb (KU)

Swiss-Prot :O75874

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB,IHC,IP

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:100|IP,1:50 - 1:200

Background :Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Sep 2013]

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :Refer to figures

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :STIM1; D11S4896E; GOK; IMD10; STRMK; TAM; TAM1; stromal interaction molecule 1

Immunogen :Recombinant fusion protein of human STIM1(NP_003147.2).

Conjugate :Unconjugated

Modification :Unmodification

Immunohistochemistry of paraffin-embedded mouse brain using STIM1 Rabbit pAb at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug STIM1 antibody . Western blot was performed from the immunoprecipitate using STIM1 antibody at a dilution of 1:1000.